Wittmann, Torsten; Bokoch, Gary M.; Waterman-Storer, Clare M. (2021). CIL:11845, Potorous tridactylus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00P0XRC

Actin dynamics in a Rac1(Q61L)-expressing PtK1 cell injected with Pak inhibitory fragment PBD/ID(H83L). Fast retrograde flow still occurs in the lamellipodium, but the width of the lamellipodium is reduced. Retrograde flow in the lamellum is almost stopped. The cell was microinjected with X-rhodamine-conjugated actin and observed by spinning disk confocal microscopy. Fluorescent speckle time-lapse series were acquired on a spinning disk confocal microscope with a 100/1.4 NA Plan Apo objective lens. Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 5B of associated manuscript (J Cell Biol, 161:845-851, 2003).

• 2021

• Bokoch, Gary M.
• Waterman-Storer, Clare M.
• Wittmann, Torsten

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: X-Rhodamine
Processing history: unprocessed raw data
Data qualification: Raw;intensitiesquantitation

• Cell Image Library Group ID: 4105

• Potorous tridactylus

• Actin cytoskeleton
• Epithelial cell

• Actin polymerization or depolymerization
• PAK inhibition
• Pt K1 (NBL-3)
• Rac activation

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 11845

Doi: https://doi.org/10.6075/J00P0XRC

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL11845

Reference

• J Cell Biol, 161:845-851, 2003. https://www.ncbi.nlm.nih.gov/pubmed/?term=12796474
• Wittmann T, Bokoch GM, Waterman-Storer CM. Regulation of leading edge microtubule and actin dynamics downstream of Rac1. J Cell Biol. 2003 Jun 9;161(5):845-51. https://doi.org/10.1083/jcb.200303082

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12