{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1293858000},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Mp4","File_path":"11845_web.mp4","Size":598518,"Mime_type":"video\/mp4"},{"File_type":"Jpeg","File_path":"11845.jpg","Size":39616,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"11845.zip","Size":5521536,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Biol, 161:845-851, 2003"],"Contributors":["Torsten Wittmann","Gary M. Bokoch","Clare M. Waterman-Storer"],"PUBMED":["12796474"]},"BIOLOGICALPROCESS":{"onto_name":"actin polymerization or depolymerization","onto_id":"GO:0008154"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"Pt K1 (NBL-3)","onto_id":"MCC:0000388"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Actin dynamics in a Rac1(Q61L)-expressing PtK1 cell injected with Pak inhibitory fragment PBD\/ID(H83L). Fast retrograde flow still occurs in the lamellipodium, but the width of the lamellipodium is reduced. Retrograde flow in the lamellum is almost stopped. The cell was microinjected with X-rhodamine-conjugated actin and observed by spinning disk confocal microscopy. Fluorescent speckle time-lapse series were acquired on a spinning disk confocal microscope with a 100\/1.4 NA Plan Apo objective lens.   Images were taken every 5 s and are played back at 15 frames\/s. For still images, see Fig. 5B of associated manuscript (J Cell Biol, 161:845-851, 2003)."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"actin cytoskeleton","onto_id":"GO:0015629"},"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"4105","MOLECULARFUNCTION":[{"free_text":"PAK inhibition"},{"free_text":"Rac activation"}],"DATAQUALIFICATION":{"free_text":"RAW;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":475,"Pixel_size":{"unit":"microns","value":0.131},"axis":"X"}},{"Space":{"Image_size":450,"Pixel_size":{"unit":"microns","value":0.131},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Potorous tridactylus","onto_id":"NCBITaxon:9310"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"X-Rhodamine","onto_id":"FBbi:00000584"},"CELLTYPE":{"onto_name":"epithelial cell","onto_id":"CL:0000066"}}},"Citation":{"Title":"Torsten Wittmann, Gary M. Bokoch, Clare M. Waterman-Storer (2011) CIL:11845, Potorous tridactylus, epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil11845","DOI":"doi:10.7295\/W9CIL11845"}}}