Myers, Kenneth; Waterman, Clare M. (2021). CIL:8951, Mus musculus, blood vessel endothelial cell, primary cell line cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J06H4GG2

Individual Mouse Aortic Endothelial Cell (MAEC) migrating in a 3-dimensional collagen gel (1.4mg/mL). Imaged on a spinning disk confocal microscope using a 100x oil immersion objective lens on a Nikon Eclipse Ti equipped with Perfect Focus System, an electronic shutter (Smart shutter, Sutter Instruments; Novato, CA) for transmitted illumination, a linear-encoded X, Y, Z robotic stage with a piezo-driven Z-axis top plate (ASI Technologies, Montgomeryville, PA) and a Yokogawa CSU-X spinning disk confocal scan head in an electronic filterwheel. Total imaging time = 1hr, 12mins.

• 2021

• Myers, Kenneth
• Waterman, Clare M.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro; cells in 3D matrix
Item type: recorded image
Imaging mode: phase contrast microscopy; spinning disk confocal microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data

• Mus musculus

• Blood vessel endothelial cell
• Cell
• Extracellular matrix
• Primary cell line cell

• Cell migration

• data
• image

• No linguistic content; Not applicable

Samplenumber: 8951

Doi: https://doi.org/10.6075/J06H4GG2

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL8951

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2022-08-12