{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1291525200},"Data_type":{"Still_image":false,"Z_stack":false,"Video":false,"Time_series":true},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"8951.zip","Size":625732028,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"8951.tif","Size":626400000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"8951.jpg","Size":309736,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"OTHER":["Laboratory of Cell and Tissue Morphodynamics, NHLBI, NIH"],"Contributors":["Kenneth Myers","Clare M. Waterman"]},"BIOLOGICALPROCESS":{"onto_name":"cell migration","onto_id":"GO:0016477"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"elastic scattering of photons","onto_id":"FBbi:00000587"},"IMAGEDESCRIPTION":{"free_text":"Individual Mouse Aortic Endothelial Cell (MAEC) migrating in a 3-dimensional collagen gel (1.4mg\/mL). Imaged on a spinning disk confocal microscope using a 100x oil immersion objective lens on a Nikon Eclipse Ti equipped with Perfect Focus System, an electronic shutter (Smart shutter, Sutter Instruments; Novato, CA) for transmitted illumination, a linear-encoded X, Y, Z robotic stage with a piezo-driven Z-axis top plate (ASI Technologies, Montgomeryville, PA) and a Yokogawa CSU-X spinning disk confocal scan head in an electronic filterwheel. Total imaging time = 1hr, 12mins."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"cell","onto_id":"GO:0005623"},{"onto_name":"extracellular matrix","onto_id":"GO:0031012"}],"RELATIONTOINTACTCELL":[{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},{"free_text":"cells in 3D matrix"}],"SOURCEOFCONTRAST":{"onto_name":"differences in amount of elastic light scattering","onto_id":"FBbi:00000600"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"phase contrast microscopy","onto_id":"FBbi:00000247"},{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"}],"DIMENSION":[{"Space":{"Image_size":1392,"Pixel_size":{"unit":"nanometers","value":105},"axis":"X"}},{"Space":{"Image_size":1040,"Pixel_size":{"unit":"nanometers","value":105},"axis":"Y"}},{}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"},"CELLTYPE":[{"onto_name":"blood vessel endothelial cell","onto_id":"CL:0000071"},{"onto_name":"primary cell line cell","onto_id":"CL:0000001"}]}},"Citation":{"Title":"Kenneth Myers, Clare M. Waterman (2010) CIL:8951, Mus musculus, blood vessel endothelial cell, primary cell line cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil8951","DOI":"doi:10.7295\/W9CIL8951"}}}