Svitkina, Tatyana M.; Verkhovsky, Alexander B.; Borisy, Gary G. (2021). CIL:23050, rattus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J05M64KR

Relative distribution of microtubuless and plectin. Electron rnicrograph shows gold-labeled plectin (10 nm) connecting an intermediate filament to microtubules when they
cross each other at different angles. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 7i from J Cell Biol. 1996 Nov;135(4):991-1007.

• 2021

• Borisy, Gary G.
• Svitkina, Tatyana M.
• Verkhovsky, Alexander B.

Preparation: glutaraldehyde fixed tissue; critical_point dried specimen; tannic acid
Relation to intact cell: platinum replica
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: uranyl salt; shadowing and plating; primary antibody plus labeled secondary antibody; immunogold
Processing history: unprocessed raw data

• Cell Image Library Group ID: 8924

• Rattus

• Fibroblast
• Intermediate filament
• Microtubule
• Plectin

• REF-52

• data
• image

• No linguistic content; Not applicable

Samplenumber: 23050

Doi: https://doi.org/10.6075/J05M64KR

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL23050

Reference

• J Cell Biol. 1996 Nov;135(4):991-1007 https://www.ncbi.nlm.nih.gov/pubmed/?term=8922382

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2022-08-12