{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1301544000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"23050.jpg","Size":4664940,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"23050.zip","Size":14710034,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"23050.tif","Size":14800000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Biol. 1996 Nov;135(4):991-1007"],"Contributors":["Tatyana M. Svitkina","Alexander B. Verkhovsky,","Gary G. Borisy"],"PUBMED":["8922382"]},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"free_text":"REF-52"},"PARAMETERIMAGED":{"onto_name":"elastic scattering of electrons","onto_id":"FBbi:00000586"},"IMAGEDESCRIPTION":{"free_text":"Relative distribution of microtubuless and plectin. Electron rnicrograph shows gold-labeled plectin (10 nm) connecting an intermediate filament to microtubules when they\ncross each other at different angles. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon.  Image corresponds to Fig 7i from J Cell Biol. 1996 Nov;135(4):991-1007."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"free_text":"plectin"},{"onto_name":"microtubule","onto_id":"GO:0005874"},{"onto_name":"intermediate filament","onto_id":"GO:0005882"}],"RELATIONTOINTACTCELL":{"free_text":"platinum replica"},"SOURCEOFCONTRAST":{"onto_name":"differences in deposition of metal shadow","onto_id":"FBbi:00000601"},"GROUP_ID":"8924","TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":4428,"axis":"X"}},{"Space":{"Image_size":3318,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"free_text":"rattus"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"free_text":"tannic acid"},{"onto_name":"critical_point dried specimen","onto_id":"FBbi:00000581"}],"VISUALIZATIONMETHODS":[{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"shadowing and plating","onto_id":"FBbi:00000398"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"free_text":"immunogold"}],"CELLTYPE":{"onto_name":"fibroblast","onto_id":"CL:0000057"}}},"Citation":{"Title":"Tatyana M. Svitkina, Alexander B. Verkhovsky,, Gary G. Borisy (2011) CIL:23050, rattus, fibroblast. CIL. Dataset","ARK":"ark:\/b7295\/w9cil23050","DOI":"doi:10.7295\/W9CIL23050"}}}