CIL:13367, Homo sapiens
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- Collection
- Cite This Work
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Porter, Iain M.; McClelland, Sarah E.; Khoudoli, Guennadi A.; Hunter, Christopher J.; Andersen, Jens S.; McAinsh, Andrew D.; Blow, J. Julian; Swedlow, Jason R. (2021). CIL:13367, Homo sapiens. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0WH2NQQ
- Description
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4D image series of HeLa cells cotransfected with CENP-B–GFP (centromeric protein B) and either control (CIL:13368) or Bod1 siRNA (this image). Bod1 is a protein that associates wtih a large macromolecular complex and localizes with kinetochores and spindle poles during mitosis. .
- Date Issued
- 2021
- Researchers
- Methods
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48 h after transfection, cells were split onto 40-mm-diameter glass coverslips (Bioptechs), cultured overnight, and transferred to CO2 independent media with supplements. Cells were maintained at 37°C using an FCS2 chamber in conjunction with an objective heater (Bioptechs). Images were acquired on a restoration microscope (DeltaVision Spectris; Applied Precision) with a 100× 1.35 NA objective and a cooled charge-coupled device camera (CoolSNAP HQ; Roper Scientific). SoftWorx software (Applied Precision) was used for image analysis. Datasets were deconvolved using the constrained iterative algorithm (Swedlow et al., 1997; Wallace et al., 2001) using SoftWorx software. Time courses were presented as maximum intensity projections of deconvolved three-dimentional datasets. Images were loaded into Photoshop (Adobe) or OMERO (http://openmicroscopy.org) and adjusted for display. Image is part of Fig 4B in JCB vol. 179 no. 2 187-197
- Technical Details
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Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: constrained iterative deconvolution - Series
- Scientific Name
- Anatomy
- Topics
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 13367
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13367
- JCB vol. 179 no. 2 187-197 https://www.ncbi.nlm.nih.gov/pubmed/?term=17938248
- Porter IM, McClelland SE, Khoudoli GA, Hunter CJ, Andersen JS, McAinsh AD, Blow JJ, Swedlow JR. Bod1, a novel kinetochore protein required for chromosome biorientation. J Cell Biol. 2007 Oct 22;179(2):187-97. https://doi.org/10.1083/jcb.200704098
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- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12