{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1303099200},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":true},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"13367.tif","Size":118700000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"13367.zip","Size":118644626,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"13367.jpg","Size":5504,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"Journal of Cell Biology","Href":"http:\/\/jcb.rupress.org\/content\/179\/2\/187.long"}],"PUBLISHED":["JCB vol. 179 no. 2 187-197"],"Contributors":["Iain M. Porter","Sarah E. McClelland","Guennadi A. Khoudoli","Christopher J. Hunter","Jens S. Andersen","Andrew D. McAinsh","J. Julian Blow","Jason R. Swedlow"],"PUBMED":["17938248"]},"BIOLOGICALPROCESS":{"onto_name":"mitosis","onto_id":"GO:0007067"},"PROCESSINGHISTORY":{"onto_name":"constrained iterative deconvolution","onto_id":"FBbi:00000360"},"CELLLINE":{"onto_name":"HeLa S3","onto_id":"MCC:0000220"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"4D image series of HeLa cells cotransfected with CENP-B\u2013GFP  (centromeric protein B) and either control (CIL:13368) or Bod1 siRNA (this image).   Bod1 is a protein that associates wtih a large macromolecular complex and localizes with kinetochores and spindle poles during mitosis.  ."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"chromosome, centromeric region","onto_id":"GO:0000775"},{"free_text":"CENP-B"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"11195","MOLECULARFUNCTION":{"free_text":"Bod1 siRNA treatment"},"TECHNICALDETAILS":{"free_text":"48 h after transfection, cells were split onto 40-mm-diameter glass coverslips (Bioptechs), cultured overnight, and transferred to CO2 independent media with supplements. Cells were maintained at 37°C using an FCS2 chamber in conjunction with an objective heater (Bioptechs). Images were acquired on a restoration microscope (DeltaVision Spectris; Applied Precision) with a 100× 1.35 NA objective and a cooled charge-coupled device camera (CoolSNAP HQ; Roper Scientific). SoftWorx software (Applied Precision) was used for image analysis. Datasets were deconvolved using the constrained iterative algorithm (Swedlow et al., 1997; Wallace et al., 2001) using SoftWorx software. Time courses were presented as maximum intensity projections of deconvolved three-dimentional datasets. Images were loaded into Photoshop (Adobe) or OMERO (http:\/\/openmicroscopy.org) and adjusted for display. Image is part of Fig 4B in JCB vol. 179 no. 2 187-197"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":237,"Pixel_size":{"unit":"microns","value":0.3312},"axis":"X"}},{"Space":{"Image_size":265,"Pixel_size":{"unit":"microns","value":0.3312},"axis":"Y"}},{"Space":{"Image_size":6,"Pixel_size":{"unit":"microns","value":2.25},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"EGFP","onto_id":"FBbi:00000082"}}},"Citation":{"Title":"Iain M. Porter, Sarah E. McClelland, Guennadi A. Khoudoli, Christopher J. Hunter, Jens S. Andersen, Andrew D. McAinsh, J. Julian Blow, Jason R. Swedlow (2011) CIL:13367, Homo sapiens. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13367","DOI":"doi:10.7295\/W9CIL13367"}}}