Goehring, Nathan W.; Hoege, Carsten; Grill, Stephan W.; Hyman, Anthony A. (2021). CIL:37933, Caenorhabditis elegans, early embryonic cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0MG7N62

Anterior and posterior regions of a C. elegans embryo are determined by PAR protein distribution. PAR-2, shown in green marks the posterior region of the embryo and PAR-6, shown in red, marks the anterior region. DNA is in blue. The embryo was treated with the drug cytochalasin D (disrupts the actin cytoskeleton) at frame 19. In the presence of the drug, PAR domains remain stable until anaphase when PAR-2 is lost to invaginations.

• 2021

• Goehring, Nathan W.
• Grill, Stephan W.
• Hoege, Carsten
• Hyman, Anthony A.

C. elegans embryo expressing mCherry::PAR-2 (green) and GFP::PAR-6 (red) was treated with cytochalasin-D. Embryo was made permeable by placing L4 larvae on F08F8.2(RNAi) plates for 20-24h. Images were captured by wide-field fluorescence microscopy (Zeiss Axioplan II, 63x/1.4 Oil PlanApochromat objective, Hamamatsu Orca-ER camera) at 30 second intervals. Time-lapse images correspond to Video1 (at 8 frames/second) and Fig 4C in J Cell Biol. 2011. 193(3): 583-594.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image; time lapse microscopy
Imaging mode: widefield illumination; fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein; compartmentalization of stain or label
Visualization methods: mCherryFP; EGFP; Hoechst
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 9659

• Caenorhabditis elegans

• Cell cortex
• Early embryonic cell

• Anterior/posterior axis specification, embryo
• Embryo development ending in birth or egg hatching
• Establishment or maintenance of polarity of embryonic epithelium

• data
• image

• No linguistic content; Not applicable

Samplenumber: 37933

Doi: https://doi.org/10.6075/J0MG7N62

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL37933

Reference

• J Cell Biol. 2011. 193(3): 583-594. https://www.ncbi.nlm.nih.gov/pubmed/?term=21518794

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

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2022-08-12