{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1320033600},"Data_type":{"Still_image":false,"Z_stack":false,"Video":false,"Time_series":true},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"37933.tif","Size":20700000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"37933.jpg","Size":12070,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"37933.zip","Size":20684847,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/4354"}],"PUBLISHED":["J Cell Biol. 2011. 193(3): 583-594."],"Contributors":["Nathan W. Goehring","Carsten Hoege","Stephan W. Grill","Anthony A. Hyman"],"PUBMED":["21518794"]},"BIOLOGICALPROCESS":[{"onto_name":"embryo development ending in birth or egg hatching","onto_id":"GO:0009792"},{"onto_name":"establishment or maintenance of polarity of embryonic epithelium","onto_id":"GO:0016332"},{"onto_name":"anterior\/posterior axis specification, embryo","onto_id":"GO:0008595"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Anterior and posterior regions of a C. elegans embryo are determined by PAR protein distribution. PAR-2, shown in green marks the posterior region of the embryo and PAR-6, shown in red, marks the anterior region. DNA is in blue. The embryo was treated with the drug cytochalasin D (disrupts the actin cytoskeleton) at frame 19. In the presence of the drug, PAR domains remain stable until anaphase when PAR-2 is lost to invaginations."},"ITEMTYPE":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"time lapse microscopy","onto_id":"FBbi:00000249"}],"CELLULARCOMPONENT":{"onto_name":"cell cortex","onto_id":"GO:0005938"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":[{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},{"onto_name":"compartmentalization of stain or label","onto_id":"FBbi:00000604"}],"GROUP_ID":"9659","TECHNICALDETAILS":{"free_text":"C. elegans embryo expressing mCherry::PAR-2 (green) and GFP::PAR-6 (red) was treated with cytochalasin-D. Embryo was made permeable by placing L4 larvae on F08F8.2(RNAi) plates for 20-24h. Images were captured by wide-field fluorescence microscopy (Zeiss Axioplan II, 63x\/1.4 Oil PlanApochromat objective, Hamamatsu Orca-ER camera) at 30 second intervals. Time-lapse images correspond to Video1 (at 8 frames\/second) and Fig 4C in J Cell Biol. 2011. 193(3): 583-594."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"widefield illumination","onto_id":"FBbi:00000277"},{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"}],"DIMENSION":[{"Space":{"Image_size":382,"Pixel_size":{"unit":"microns","value":0.205},"axis":"X"}},{"Space":{"Image_size":360,"Pixel_size":{"unit":"microns","value":0.205},"axis":"Y"}},{"Time":{"unit":"seconds","value":30,"frame":"50"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Caenorhabditis elegans","onto_id":"NCBITaxon:6239"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":[{"onto_name":"mCherryFP","onto_id":"FBbi:00000525"},{"onto_name":"EGFP","onto_id":"FBbi:00000082"},{"free_text":"Hoechst"}],"CELLTYPE":{"onto_name":"early embryonic cell","onto_id":"CL:0000007"}}},"Citation":{"Title":"Nathan W. Goehring, Carsten Hoege, Stephan W. Grill, Anthony A. Hyman (2011) CIL:37933, Caenorhabditis elegans, early embryonic cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil37933","DOI":"doi:10.7295\/W9CIL37933"}}}