Allen, Richard (2021). CIL:7749, Nassula, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0GX49KZ

Transmission electron micrograph of section through the macronucleus of Nassula sp containing many darkly staining nucleoli.

• 2021

• Allen, Richard

TEM taken on 4/1/69 by R. Allen with Philips 300 operating at 60kV. Neg. 7,650X. Bar = 1µm. A print of the negative was scanned and processed in Photoshop. This image is suitable for quantitative analysis. A lower resolution image (CIL:11039) is available in the library for qualitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydration in alcohol and embedding in epoxy resin. Microtome section thickness approximately 75nm.

Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 11218

• Nassula

• Cell by organism
• Ciliated Protist
• Eukaryotic cell
• Eukaryotic Protist
• Macronucleus
• Nucleolus
• Nucleolus organizer region

• Cambridge Culture Collection, Cambridge, England, catalog # LB1650/1
• Macronucleus organization
• Nucleolus organization
• Nucleolus to nucleoplasm transport

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7749

Doi: https://doi.org/10.6075/J0GX49KZ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7749

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12