{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1295326800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"7749.jpg","Size":9865564,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"7749.tif","Size":62500000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"7749.zip","Size":64859854,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Allen"]},"BIOLOGICALPROCESS":[{"onto_name":"macronucleus organization","onto_id":"GO:0032124"},{"onto_name":"nucleolus organization","onto_id":"GO:0007000"},{"onto_name":"nucleolus to nucleoplasm transport","onto_id":"GO:0032066"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"}],"CELLLINE":{"free_text":"Cambridge Culture Collection, Cambridge, England, catalog # LB1650\/1"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Transmission electron micrograph of section through the macronucleus of Nassula sp  containing many darkly staining nucleoli."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"macronucleus","onto_id":"GO:0031039"},{"onto_name":"nucleolus","onto_id":"GO:0005730"},{"onto_name":"nucleolus organizer region","onto_id":"GO:0005731"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"11218","TECHNICALDETAILS":{"free_text":"TEM taken on 4\/1\/69 by R. Allen with Philips 300 operating at 60kV. Neg. 7,650X. Bar = 1µm. A print of the negative was scanned and processed in Photoshop. This image is suitable for quantitative analysis. A lower resolution image (CIL:11039) is available in the library for qualitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydration in alcohol and embedding in epoxy resin. Microtome section thickness approximately 75nm.\n\nAdditional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":5024,"Pixel_size":{"unit":"nanometers","value":2},"axis":"X"}},{"Space":{"Image_size":6206,"Pixel_size":{"unit":"nanometers","value":2},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Nassula","onto_id":"NCBITaxon:502083"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (2011) CIL:7749, Nassula, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil7749","DOI":"doi:10.7295\/W9CIL7749"}}}