Worth, Daniel C.; Hodivala-Dilke, Kairbaan; Robinson, Stephen D.; King, Samantha J.; Morton, Penny E.; Gertler, Frank B.; Humphries, Martin J.; Parsons, Maddy (2021). CIL:12285, Mus musculus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0KK99JW

This confocal image of beta3-/- integrin cells plated on cell-derived matrices has an accompanying image of the wild type control cells. There are ncreased numbers of peripheral matrix-associated adhesions in the beta3-/- cells. The cells are co-stained for active beta1 integrin (9EG7-FITC, green), paxillin (Alexa-568, red) and fibronectin (Alexa-633, blue). Images were acquired using a Zeiss LSM 510 Meta microscope, 1024x1024 resolution, 13 stacks in the Z plane at 0.5um intervals. Images correspond to Fig 1C in J Cell Bio. 2010. 189:369-383

• 2021

• Gertler, Frank B.
• Hodivala-Dilke, Kairbaan
• Humphries, Martin J.
• King, Samantha J.
• Morton, Penny E.
• Parsons, Maddy
• Robinson, Stephen D.
• Worth, Daniel C.

Preparation: chemically fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Fluorescein (FITC); Alexa Fluor 568; Alexa Fluor 633; primary antibody plus labeled secondary antibody
Processing history: color combine
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 3605

• Mus musculus

• Cell derived 3D matrix
• Extracellular matrix
• Fibroblast
• Focal adhesion

• Migration in 3D matrix
• Wild type corresponding tobeta3 integrin -/-

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12285

Doi: https://doi.org/10.6075/J0KK99JW

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12285

Reference

• J Cell Bio 2010. 189:369-383 https://www.ncbi.nlm.nih.gov/pubmed/?term=20404115

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2022-08-12