{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1295154000},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"12285.tif","Size":54600000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"12285.zip","Size":54594460,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"12285.jpg","Size":208144,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Bio 2010. 189:369-383"],"Contributors":["Daniel C. Worth","Kairbaan Hodivala-Dilke","Stephen D. Robinson","Samantha J. King","Penny E. Morton","Frank B. Gertler","Martin J. Humphries","Maddy Parsons"],"PUBMED":["20404115"]},"BIOLOGICALPROCESS":{"free_text":"migration in 3D matrix"},"PROCESSINGHISTORY":{"free_text":"color combine"},"CELLLINE":{"free_text":"wild type corresponding tobeta3 integrin -\/-"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"This confocal image of beta3-\/- integrin cells plated on cell-derived matrices has an accompanying image of the wild type control cells.  There are ncreased numbers of peripheral matrix-associated adhesions in the beta3-\/- cells. The cells are co-stained for active  beta1 integrin (9EG7-FITC, green), paxillin (Alexa-568, red) and fibronectin (Alexa-633, blue). Images were acquired using a Zeiss LSM 510 Meta microscope, 1024x1024 resolution, 13 stacks in the Z plane at 0.5um intervals.  Images correspond to Fig 1C in  J Cell Bio. 2010. 189:369-383"},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"focal adhesion","onto_id":"GO:0005925"},{"onto_name":"extracellular matrix","onto_id":"GO:0031012"},{"free_text":"cell derived 3D matrix"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"3605","DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"single-spot confocal microscopy","onto_id":"FBbi:00000252"},"DIMENSION":[{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.127},"axis":"X"}},{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.127},"axis":"Y"}},{"Wavelength":{"unit":"microns","value":"488, 561, 633"}},{"Space":{"Pixel_size":{"unit":"microns","value":0.5},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"chemically fixed tissue","onto_id":"FBbi:00000002"},"VISUALIZATIONMETHODS":[{"onto_name":"Fluorescein (FITC)","onto_id":"FBbi:00000451"},{"onto_name":"Alexa Fluor 568","onto_id":"FBbi:00000442"},{"onto_name":"Alexa Fluor 633","onto_id":"FBbi:00000446"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"}],"CELLTYPE":{"onto_name":"fibroblast","onto_id":"CL:0000057"}}},"Citation":{"Title":"Daniel C. Worth, Kairbaan Hodivala-Dilke, Stephen D. Robinson, Samantha J. King, Penny E. Morton, Frank B. Gertler, Martin J. Humphries, Maddy Parsons (2011) CIL:12285, Mus musculus, fibroblast. CIL. Dataset","ARK":"ark:\/b7295\/w9cil12285","DOI":"doi:10.7295\/W9CIL12285"}}}