CIL:111, Mus musculus, sensory neuron
CIL source metadata (JSON)
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- Collection
- Cite This Work
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Parysek, Linda (2021). CIL:111, Mus musculus, sensory neuron. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00R9NMC
- Description
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This is a section of methanol-fixed, cryostat-sectioned (10 micrometers thick) adult mouse cervical dorsal root ganglion, labeled with antibodies to peripherin (green) and neurofilament protein NF-M (red) components of the intermediate filament cytoskeleton. Note that small diameter neurons and small-caliber fibers, seen in the lower half of the image, are enriched in peripherin. Large-size neurons and thick-caliber fibers are enriched in NF proteins.
Microscope - Zeiss Axioplan I
Illumination - Mercury arc lamp
Objective - 40X oil/NA 0.9
Intermediate lens between obj and camera - 0.63X
Camera - Axiocam MRM CCD
Filters:
1. Ex 485/20 BP, Em 515-565 BP
2. Ex 560/40, Em 630/60 - Date Issued
- 2021
- Researcher
- Technical Details
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Preparation: coagulating-fixative fixed tissue
Relation to intact cell: cryostat-sectioned tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Alexa Fluor 488; Alexa Fluor 594; primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw - Scientific Name
- Anatomy
- Topics
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 111
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL111
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12