{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1293858000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"111.jpg","Size":297480,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"111.zip","Size":4021478,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"111.tif","Size":4100000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"Contributors":["Linda Parysek"]},"BIOLOGICALPROCESS":{"onto_name":"intermediate filament-based process","onto_id":"GO:0045103"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"This is a section of methanol-fixed, cryostat-sectioned (10 micrometers thick) adult mouse cervical dorsal root ganglion, labeled with antibodies to peripherin (green) and neurofilament protein NF-M (red) components of the intermediate filament cytoskeleton.  Note that small diameter neurons and small-caliber fibers, seen in the lower half of the image, are enriched in peripherin.  Large-size neurons and thick-caliber fibers are enriched in NF proteins.\n\nMicroscope - Zeiss Axioplan I\nIllumination - Mercury arc lamp\nObjective - 40X oil\/NA 0.9\nIntermediate lens between obj and camera - 0.63X\nCamera - Axiocam MRM CCD\nFilters:\n1.  Ex 485\/20 BP, Em 515-565 BP\n2.  Ex 560\/40, Em 630\/60"},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"neurofilament","onto_id":"GO:0005883"},"RELATIONTOINTACTCELL":{"onto_name":"cryostat-sectioned tissue","onto_id":"FBbi:00000027"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"MOLECULARFUNCTION":{"onto_name":"structural constituent of cytoskeleton","onto_id":"GO:0005200"},"DATAQUALIFICATION":{"free_text":"RAW"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":1300,"Pixel_size":{"unit":"nanometers","value":260},"axis":"Y"}},{"Space":{"Image_size":1030,"Pixel_size":{"unit":"nanometers","value":260},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"coagulating-fixative fixed tissue","onto_id":"FBbi:00000003"},"VISUALIZATIONMETHODS":[{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"},{"onto_name":"Alexa Fluor 594","onto_id":"FBbi:00000444"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"}],"CELLTYPE":{"onto_name":"sensory neuron","onto_id":"CL:0000101"}}},"Citation":{"Title":"Linda Parysek (2011) CIL:111, Mus musculus, sensory neuron. CIL. Dataset","ARK":"ark:\/b7295\/w9cil111","DOI":"doi:10.7295\/W9CIL111"}}}