Allen, Richard (2021). CIL:36253, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0VD6XD5

The 11 micrographs of the group are views of a serially-sectioned contracted Vorticella convallaria cell that show the main features of this cell. This figure shows 1 food vacuole; a segment of the macronucleus; the micronucleus; the contractile vacuole, linked by CV pore to the bacteria-filled chamber; peristome; perioral cilia now closed to the outside. A sand grain (shattered during sectioning) was trapped against the cell by an unknown layer of unstained material. TEM taken on 3/17/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 3,000X. Bar = 2µm. The negative was printed to paper and the image was scanned to Photoshop. This image is suitable for qualitative analysis. A high resolution version of this image in the library (CIL:40573) is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed

• Cell Image Library Group ID: 11189

• Vorticella convallaria

• Cell
• Cell by organism
• Ciliated Protist
• Contractile vacuole
• Contractile vacuole pore
• Eukaryotic cell
• Eukaryotic Protist
• Food vacuole
• Macronucleus
• Micronucleus
• Oral apparatus

• Cambridge Culture Collection, Cambridge, England, # LB1690/1
• Contractile vacuole organization
• Cortical cytoskeleton organization
• Cytoplasm organization
• Digestive system process
• Macronucleus organization
• Micronucleus organization
• Oral apparatus organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 36253

Doi: https://doi.org/10.6075/J0VD6XD5

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36253

CC0 1.0 Universal Public Domain Dedication

Creative Commons Public Domain Dedication

Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

Constraint(s) on Use: This work may be used without prior permission.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12