{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1313380800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"36253.tif","Size":9900000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"36253.jpg","Size":2189894,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"36253.zip","Size":20005042,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Allen (University of Hawaii)"]},"BIOLOGICALPROCESS":[{"onto_name":"digestive system process","onto_id":"GO:0022600"},{"onto_name":"cytoplasm organization","onto_id":"GO:0007028"},{"onto_name":"cortical cytoskeleton organization","onto_id":"GO:0030865"},{"onto_name":"macronucleus organization","onto_id":"GO:0032124"},{"onto_name":"micronucleus organization","onto_id":"GO:0032125"},{"onto_name":"contractile vacuole organization","onto_id":"GO:0033298"},{"onto_name":"oral apparatus organization","onto_id":"GO:0032122"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"free_text":"Print from negative scanned for Photoshop."}],"CELLLINE":{"free_text":"Cambridge Culture Collection, Cambridge, England, # LB1690\/1"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"The 11 micrographs of the group are views of a serially-sectioned contracted Vorticella convallaria cell that show the main features of this cell. This figure shows 1 food vacuole; a segment of the macronucleus; the micronucleus; the contractile vacuole, linked by CV pore to the bacteria-filled chamber; peristome; perioral cilia now closed to the outside. A sand grain (shattered during sectioning) was trapped against the cell by an unknown layer of unstained material. TEM taken on 3\/17\/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 3,000X. Bar = 2µm. The negative was printed to paper and the image was scanned to Photoshop. This image is suitable for qualitative analysis. A high resolution version of this image in the library (CIL:40573) is available for quantitative analysis. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"cell","onto_id":"GO:0005623"},{"onto_name":"food vacuole","onto_id":"GO:0020020"},{"onto_name":"macronucleus","onto_id":"GO:0031039"},{"onto_name":"micronucleus","onto_id":"GO:0031040"},{"onto_name":"contractile vacuole","onto_id":"GO:0000331"},{"onto_name":"contractile vacuole pore","onto_id":"GO:0031913"},{"onto_name":"oral apparatus","onto_id":"GO:0031912"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"11189","TECHNICALDETAILS":{"free_text":"Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":3054,"axis":"X"}},{"Space":{"Image_size":3216,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Vorticella convallaria","onto_id":"NCBITaxon:60850"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (University of Hawaii) (2011) CIL:36253, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil36253","DOI":"doi:10.7295\/W9CIL36253"}}}