Gluenz, Eva (2021). CIL:12401, Leishmania mexicana, Mus musculus, macrophage, protozoan parasites. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J01V5CQ7

Leishmania mexicana amastigotes in their parasitophorous vacuole in a mouse macrophage. Murine macrophages (cell line J774) were infected in vitro with Leishmania mexicana stationary phase promastigotes (WHO strain MNYC/BZ/62/M379). Four days post infection the cells were fixed with glutaraldehyde, post-fixed with osmium tetroxide, dehydrated in ethanol and embedded in epoxy resin. Thin sections (~90 nm) were viewed on a Philips Technai 12 transmission electron microscope operating at 80 kV. Magnification 2900X.

• 2021

• Gluenz, Eva

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data
Data qualification: Processed;spatialmeasurements

• Cell Image Library Group ID: 3664

• Leishmania mexicana
• Mus musculus

• Macrophage
• Parasite-containing vacuole
• Protozoan parasites

• Amastigote form Leishmania mexicana
• J774
• Parasite entry into host cell forming a parasitophorous vacuole
• WHO strain MNYC/BZ/62/M379

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12401

Doi: https://doi.org/10.6075/J01V5CQ7

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12401

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12