{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1296018000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"12401.tif","Size":23500000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"12401.zip","Size":23425806,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"12401.jpg","Size":9090586,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Eva Gluenz"]},"BIOLOGICALPROCESS":[{"free_text":"amastigote form Leishmania mexicana"},{"free_text":"parasite entry into host cell forming a parasitophorous vacuole"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":[{"free_text":"WHO strain MNYC\/BZ\/62\/M379"},{"free_text":"J774"}],"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Leishmania mexicana amastigotes in their parasitophorous vacuole in a mouse macrophage. Murine macrophages (cell line J774) were infected in vitro with Leishmania mexicana stationary phase promastigotes (WHO strain MNYC\/BZ\/62\/M379). Four days post infection the cells were fixed with glutaraldehyde, post-fixed with osmium tetroxide, dehydrated in ethanol and embedded in epoxy resin. Thin sections (~90 nm) were viewed on a Philips Technai 12 transmission electron microscope operating at 80 kV. Magnification 2900X."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"free_text":"parasite-containing vacuole"},"RELATIONTOINTACTCELL":{"onto_name":"sectioned tissue","onto_id":"FBbi:00000026"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"3664","DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":5112,"Pixel_size":{"unit":"nanometers","value":3.28},"axis":"X"}},{"Space":{"Image_size":4576,"Pixel_size":{"unit":"nanometers","value":3.28},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":[{"onto_name":"Leishmania mexicana","onto_id":"NCBITaxon:5665"},{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"}],"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"}],"VISUALIZATIONMETHODS":{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},"CELLTYPE":[{"free_text":"protozoan parasites"},{"onto_name":"macrophage","onto_id":"CL:0000235"}]}},"Citation":{"Title":"Eva Gluenz (2011) CIL:12401, Leishmania mexicana, Mus musculus, macrophage, protozoan parasites. CIL. Dataset","ARK":"ark:\/b7295\/w9cil12401","DOI":"doi:10.7295\/W9CIL12401"}}}