CIL:25301, uncultured Scuticociliatia, Ancistrum sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Collection
- Cite This Work
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Antipa, Gregory (2021). CIL:25301, uncultured Scuticociliatia, Ancistrum sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0TX3D9B
- Description
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Ancistrum is a marine ciliate that inhabits the mantle cavity of mytiliid mussels. This image is a slightly higher resolution scan of CIL:24921 that shows a good view of the macronucleus and what are probably flask-shaped endosomes. This group of images is located in a part of the cytoplasm that especially contains these vesicles, and this image has a view at an appropriate angle to exhibit what may be dynamin or a dynamin-like protein decorating the tube of the flask shaped organelle. In addition, the macronuclear membrane is well demonstrated. TEM taken by G. Antipa in 1969 on a Hitachi HU11A operating at 75kV. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 60nm thickness.
- Date Issued
- 2021
- Researcher
- Technical Details
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Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements - Series
- Scientific Names
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 25301
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL25301
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12