{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1301630400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"25301.tif","Size":33700000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"25301.jpg","Size":4730744,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"25301.zip","Size":33661042,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Gregory Antipa (San Francisco State University)"]},"BIOLOGICALPROCESS":[{"onto_name":"cytoplasm organization","onto_id":"GO:0007028"},{"onto_name":"endosome localization","onto_id":"GO:0032439"},{"onto_name":"macronucleus organization","onto_id":"GO:0032124"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"}],"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Ancistrum is a marine ciliate that inhabits the mantle cavity of mytiliid mussels. This image is a slightly higher resolution scan of CIL:24921 that shows a good view of the macronucleus and what are probably flask-shaped endosomes. This group of images is located in a part of the cytoplasm that especially contains these vesicles, and this image has a view at an appropriate angle to exhibit what may be dynamin or a dynamin-like protein decorating the tube of the flask shaped organelle. In addition, the macronuclear membrane is well demonstrated. TEM taken by G. Antipa in 1969 on a Hitachi HU11A operating at 75kV. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 60nm thickness."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"macronucleus","onto_id":"GO:0031039"},{"onto_name":"cytoplasm","onto_id":"GO:0005737"},{"onto_name":"peroxisome","onto_id":"GO:0005777"},{"onto_name":"endosome","onto_id":"GO:0005768"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"11095","DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":6487,"Pixel_size":{"unit":"nanometers","value":1.3},"axis":"X"}},{"Space":{"Image_size":2594,"Pixel_size":{"unit":"nanometers","value":1.3},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":[{"onto_name":"uncultured Scuticociliatia","onto_id":"NCBITaxon:547477"},{"free_text":"Ancistrum sp."}],"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Gregory Antipa (San Francisco State University) (2011) CIL:25301, uncultured Scuticociliatia, Ancistrum sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil25301","DOI":"doi:10.7295\/W9CIL25301"}}}