Allen, Richard (2021). CIL:39128, Nassula, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0542MZ4

Cytopharyngeal basket in tangential section is composed of a complex of microtubular rods. The lumen of the basket contains many vesicles called cytopharyngeal vesicles. They may provide membrane for the nascent food vacuole, lysosomal and/or lytic enzymes, or all of these. Another role may be to provide vacuolar type ATPases (proton pumps) to the nascent food vacuole. TEM taken on 3/13/69 by R. Allen with Philips 300 operating at 60kV. Neg. 3,570X.

• 2021

• Allen, Richard

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 9947

• Nassula

• Cell by organism
• Ciliated Protist
• Eukaryotic cell
• Eukaryotic Protist
• Microtubule associated complex
• Oral apparatus
• Symbiont-containing vacuole

• Bacterial symbiont interaction
• Cambridge Culture Collection, Cambridge, England, catalog # LB1650/1
• Microtubule bundle formation
• Oral apparatus organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39128

Doi: https://doi.org/10.6075/J0542MZ4

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39128

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12