{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1322024400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"39128.jpg","Size":4047580,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"39128.tif","Size":35500000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Allen"]},"BIOLOGICALPROCESS":[{"onto_name":"oral apparatus organization","onto_id":"GO:0032122"},{"onto_name":"microtubule bundle formation","onto_id":"GO:0001578"},{"free_text":"bacterial symbiont interaction"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"}],"CELLLINE":{"free_text":"Cambridge Culture Collection, Cambridge, England, catalog # LB1650\/1"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Cytopharyngeal basket in tangential section is composed of a complex of microtubular rods. The lumen of the basket contains many vesicles called cytopharyngeal vesicles. They may provide membrane for the nascent food vacuole, lysosomal and\/or lytic enzymes, or all of these. Another role may be to provide vacuolar type ATPases (proton pumps) to the nascent food vacuole. TEM taken on 3\/13\/69 by R. Allen with Philips 300 operating at 60kV. Neg. 3,570X."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"oral apparatus","onto_id":"GO:0031912"},{"onto_name":"microtubule associated complex","onto_id":"GO:0005875"},{"onto_name":"symbiont-containing vacuole","onto_id":"GO:0020003"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"9947","TECHNICALDETAILS":{"free_text":"Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":4000,"Pixel_size":{"unit":"nanometers","value":1.4},"axis":"X"}},{"Space":{"Image_size":2948,"Pixel_size":{"unit":"nanometers","value":1.4},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Nassula","onto_id":"NCBITaxon:502083"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (2011) CIL:39128, Nassula, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39128","DOI":"doi:10.7295\/W9CIL39128"}}}