Doyle, Andrew D.; Wang, Francis W.; Matsumoto, Kazue; Yamada, Kenneth M. (2021). CIL:13159, Mus musculus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0TT4PNG

Multiple NIH-3T3 fibroblasts undergoing 1D fibrillar migration. Cells were plated on polyvinyl alchohol (PVA) coated coverslips that had been patterned by ablation with a 2 photon confocal microscope. Following gluteraldehyde quenching, extracellular matrix proteins were absorbed onto the PVA surface. Movie is video 3 from JCB 2009, 184:481-490

• 2021

• Doyle, Andrew D.
• Matsumoto, Kazue
• Wang, Francis W.
• Yamada, Kenneth M.

Videos were recorded on a Axiovert 135TV (Carl Zeiss, Inc.) with a charge-coupled device camera (ORCA II ER; Hamamatsu Photonics). MetaMorph imaging software was used to acquire images and control all hardware. A custom environmental chamber (Lucite) enclosed both time-lapse and TIRF microscopes and maintained cells at 37°C with 10% CO2. Images were collected every 4 min and shown at 15 frames per second.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Processing history: custom-made smoothing, sharpening, and convolution filters using MetaMorph software
Data qualification: Processed

• Cell Image Library Group ID: 9565

• Mus musculus

• Cell
• Fibroblast

• Cell migration
• NIH/3T3
• Substrate-dependent cell migration, cell attachment to substrate

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13159

Doi: https://doi.org/10.6075/J0TT4PNG

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13159

Reference

• JCB 2009, 184:481-490 https://www.ncbi.nlm.nih.gov/pubmed/?term=19221195

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2022-08-12