{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1297486800},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"13159.jpg","Size":14537,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Mp4","File_path":"13159_web.mp4","Size":728930,"Mime_type":"video\/mp4"},{"File_type":"Zip","File_path":"13159.zip","Size":430208,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["JCB 2009, 184:481-490"],"Contributors":["Andrew D. Doyle","Francis W. Wang","Kazue Matsumoto","Kenneth M. Yamada"],"PUBMED":["19221195"]},"BIOLOGICALPROCESS":[{"onto_name":"cell migration","onto_id":"GO:0016477"},{"onto_name":"substrate-dependent cell migration, cell attachment to substrate","onto_id":"GO:0006931"}],"PROCESSINGHISTORY":{"free_text":"custom-made smoothing, sharpening, and convolution filters using MetaMorph software"},"CELLLINE":{"onto_name":"NIH\/3T3","onto_id":"MCC:0000362"},"PARAMETERIMAGED":{"onto_name":"elastic scattering of photons","onto_id":"FBbi:00000587"},"IMAGEDESCRIPTION":{"free_text":"Multiple NIH-3T3 fibroblasts undergoing 1D fibrillar migration. Cells were plated on polyvinyl alchohol (PVA) coated coverslips that had been patterned by ablation with a 2 photon confocal microscope.  Following gluteraldehyde quenching, extracellular matrix proteins were absorbed onto the PVA surface.  Movie is video 3 from JCB 2009, 184:481-490"},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"cell","onto_id":"GO:0005623"},"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"differences in amount of elastic light scattering","onto_id":"FBbi:00000600"},"GROUP_ID":"9565","TECHNICALDETAILS":{"free_text":"Videos were recorded on a Axiovert 135TV (Carl Zeiss, Inc.) with a charge-coupled device camera (ORCA II ER; Hamamatsu Photonics). MetaMorph imaging software was used to acquire images and control all hardware. A custom environmental chamber (Lucite) enclosed both time-lapse and TIRF microscopes and maintained cells at 37°C with 10% CO2.  Images were collected every 4 min and shown at 15 frames per second."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"phase contrast microscopy","onto_id":"FBbi:00000247"},"DIMENSION":[{"Space":{"Image_size":694,"Pixel_size":{"unit":"microns","value":1.11},"axis":"X"}},{"Space":{"Image_size":140,"Pixel_size":{"unit":"microns","value":1.11},"axis":"Y"}},{"Time":{"unit":"seconds","value":240}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"},"CELLTYPE":{"onto_name":"fibroblast","onto_id":"CL:0000057"}}},"Citation":{"Title":"Andrew D. Doyle, Francis W. Wang, Kazue Matsumoto, Kenneth M. Yamada (2011) CIL:13159, Mus musculus, fibroblast. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13159","DOI":"doi:10.7295\/W9CIL13159"}}}