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CIL:35618, Homo sapiens, keratinocyte

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Cite This Work

Wheeler, Ann (2021). CIL:35618, Homo sapiens, keratinocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J02J69VS

Description

The relationship between the actin cytoskeleton (blue), adherens junctions (green) and phospho-Myosin Light Chain (MLC) (red) after 30 minutes of calcium stimulation.

Date Issued
  • 2021
Researcher
Methods

The specimen was fixed with labelled with 4% paraformaldehyde and labeled with AlexaFluor488 phalloidin, rabbit anti alpha-catenin primary antibodies and Cy5 anti-rabbit secondary antibodies, mouse anti Phospho-MLC Ser18/Thr19 antibodies (Cell Signaling technologies) and mouse anti cy2 secondary antibodies. Images were acquired on a Zeiss LSM510 inverted microscope using a 63x 1.4 Numeric aperture oil immersion objective.

Technical Details

Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody; phalloidin; Alexa Fluor 488; Cy2; Cy5
Processing history: unprocessed raw data

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Identifier

Samplenumber: 35618

Doi: https://doi.org/10.6075/J02J69VS

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License

CC0 1.0 Universal Public Domain Dedication

Copyright

Creative Commons Public Domain Dedication

Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

Constraint(s) on Use: This work may be used without prior permission.

Digital Object Made Available By

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

Last Modified

2022-08-12