{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1314158400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"35618.zip","Size":2271352,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"35618.jpg","Size":271541,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"35618.tif","Size":2300000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"OTHER":["Queen Mary University London","Advanced Light Microscopy Facility"],"Contributors":["Ann Wheeler"]},"BIOLOGICALPROCESS":[{"free_text":"phosphorylation of myosin light chain"},{"free_text":"calcium stimulation"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"The relationship between the actin cytoskeleton (blue), adherens junctions (green) and phospho-Myosin Light Chain (MLC) (red) after 30 minutes of calcium stimulation."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"actin filament","onto_id":"GO:0005884"},{"free_text":"myosin light chain"},{"free_text":"alpha catenin"},{"onto_name":"adherens junction","onto_id":"GO:0005912"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"},"TECHNICALDETAILS":{"free_text":"The specimen was fixed with labelled with 4% paraformaldehyde and labeled with AlexaFluor488 phalloidin, rabbit anti alpha-catenin primary antibodies and Cy5 anti-rabbit secondary antibodies, mouse anti Phospho-MLC Ser18\/Thr19 antibodies (Cell Signaling technologies) and mouse anti cy2 secondary antibodies. Images were acquired on a Zeiss LSM510 inverted microscope using a 63x 1.4 Numeric aperture oil immersion objective."},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"single-spot confocal microscopy","onto_id":"FBbi:00000252"},"DIMENSION":[{"Space":{"Image_size":740,"Pixel_size":{"unit":"microns","value":0.143},"axis":"X"}},{"Space":{"Image_size":1023,"Pixel_size":{"unit":"microns","value":0.143},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":[{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"onto_name":"phalloidin","onto_id":"FBbi:00000100"},{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"},{"onto_name":"Cy2","onto_id":"FBbi:00000448"},{"onto_name":"Cy5","onto_id":"FBbi:00000450"}],"CELLTYPE":{"onto_name":"keratinocyte","onto_id":"CL:0000312"}}},"Citation":{"Title":"Ann Wheeler (2011) CIL:35618, Homo sapiens, keratinocyte. CIL. Dataset","ARK":"ark:\/b7295\/w9cil35618","DOI":"doi:10.7295\/W9CIL35618"}}}