Fink, Rachel; Wadsworth, Pat (2021). CIL:35203, Fundulus heteroclitus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0QZ28MR

This video shows the dynamics of the apical cell surface of individual cells of the enveloping layer in a killifish embryo. The fluorescent actin is seen to polymerize and depolymerize in cyclic waves that extend and retract these surface protrusions. Two neighboring cells from a continuous epithelium are expressing the fluorescent GFP-actin. The expression of this inserted gene is mosaic, with some cells expressing, and others not.

• 2021

• Fink, Rachel
• Wadsworth, Pat

Visualizations taken from intact embryos, injected with GFP-actin plasmid at 1-cell stage. Filmed during gastrulation.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data

• Cell Image Library Group ID: 9029

• Fundulus heteroclitus

• Actin cytoskeleton
• Epithelial cell

• Cytoskeleton organization

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 35203

Doi: https://doi.org/10.6075/J0QZ28MR

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35203

Other resource

• Rachael Fink videos (rvideo5.htm): https://www.mtholyoke.edu/people/rachel-fink/rvideo5.htm

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

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2022-08-12