{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1314158400},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"35203.jpg","Size":15585,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Mp4","File_path":"35203_web.mp4","Size":306619,"Mime_type":"video\/mp4"},{"File_type":"Zip","File_path":"35203.zip","Size":430208,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"OTHER":["Zebrafish -- The Living Laboratory"],"URLs":[{"Label":"Rachel Fink videos","Href":"http:\/\/www.mtholyoke.edu\/courses\/rfink\/Researchvideopages\/rvideo6.htm"}],"Contributors":["Rachel Fink","Pat Wadsworth"]},"BIOLOGICALPROCESS":{"onto_name":"cytoskeleton organization","onto_id":"GO:0007010"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"This video shows the dynamics of the apical cell surface of individual cells of the enveloping layer in a killifish embryo. The fluorescent actin is seen to polymerize and depolymerize in cyclic waves that extend and retract these surface protrusions. Two neighboring cells from a continuous epithelium are expressing the fluorescent GFP-actin. The expression of this inserted gene is mosaic, with some cells expressing, and others not."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"actin cytoskeleton","onto_id":"GO:0015629"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"9029","TECHNICALDETAILS":{"free_text":"Visualizations taken from intact embryos, injected with GFP-actin plasmid at 1-cell stage. Filmed during gastrulation."},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":324,"axis":"X"}},{"Space":{"Image_size":240,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Fundulus heteroclitus","onto_id":"NCBITaxon:8078"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"EGFP","onto_id":"FBbi:00000082"},"CELLTYPE":{"onto_name":"epithelial cell","onto_id":"CL:0000066"}}},"Citation":{"Title":"Rachel Fink, Pat Wadsworth (2011) CIL:35203, Fundulus heteroclitus, epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil35203","DOI":"doi:10.7295\/W9CIL35203"}}}