Salmon, Wendy C.; Adams, Michael C.; Waterman-Storer, Clare M. (2021). CIL:11932, Taricha granulosa, epithelial cell of lung. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0W66JH6

Four general zones of f-actin movement are seen in primary cultures of newt lung epithelial cells microinjected with X-rhodamine actin. At the leading edge and throughout the lamellipodium, f-actin speckles continuously appeared and moved towards the cell center. As speckles approached the junction between the lamellipodium and lamellum they usually disappeared, F-actin underwent slower retrograde flow in the lamellum, and F-actin generally moved forward in the cell body. Time-lapse FSM was performed on a spinning disk confocal microscope system. Light from a 50 mW Krypton-Argon ion laser (Melles Griot, OmniChrome) was delivered by a single-mode fiber optic (Point Source) to a Yokogawa spinning disk confocal scan-head (Ultra-View; PerkinElmer) on an inverted microscope (TE300 Quantum; Nikon). Images were collected by a 100x 1.4NA Plan-Apo DIC objective lens (Nikon) and captured with an Orca 2 camera (Hamamatsu). Images were collected in the ventral focal plane at 10 s intervals. Images were processed as follows: (1) background subtraction; (2) 3 x3 low pass filter; (3) unsharp mask filter. Corresponds to Fig 2E and video 2 in JCB , Volume 158, Number 1, July 8, 2002 31-37

• 2021

• Adams, Michael C.
• Salmon, Wendy C.
• Waterman-Storer, Clare M.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: spinning disk confocal microscopy; fluorescent speckle microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: X-Rhodamine
Processing history: background subtraction, 3X3 low pass filter, unsharp mask
Data qualification: Processed;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 3420

• Taricha granulosa

• Actin cytoskeleton
• Epithelial cell of lung

• Actin filament depolymerization
• Actin filament polymerization

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 11932

Doi: https://doi.org/10.6075/J0W66JH6

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL11932

Reference

• J Cell Biol, 158:31-37, 2002 https://www.ncbi.nlm.nih.gov/pubmed/?term=12105180
• Salmon WC, Adams MC, Waterman-Storer CM. Dual-wavelength fluorescent speckle microscopy reveals coupling of microtubule and actin movements in migrating cells. J Cell Biol. 2002 Jul 8;158(1):31-7. https://doi.org/10.1083/jcb.200203022

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2022-08-12