{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1295067600},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"11932.zip","Size":7938176,"Mime_type":"application\/zip"},{"File_type":"Mp4","File_path":"11932_web.mp4","Size":786278,"Mime_type":"video\/mp4"},{"File_type":"Jpeg","File_path":"11932.jpg","Size":34464,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Biol, 158:31-37, 2002"],"Contributors":["Wendy C. Salmon","Michael C. Adams","Clare M. Waterman-Storer"],"PUBMED":["12105180"]},"BIOLOGICALPROCESS":[{"onto_name":"actin filament polymerization","onto_id":"GO:0030041"},{"onto_name":"actin filament depolymerization","onto_id":"GO:0030042"}],"PROCESSINGHISTORY":{"free_text":"background subtraction, 3X3 low pass filter, unsharp mask"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Four general zones of f-actin movement are seen in primary cultures of newt lung epithelial cells microinjected with X-rhodamine actin.  At the leading edge and throughout the lamellipodium, f-actin speckles continuously appeared and moved towards the cell center.  As speckles approached the junction between the lamellipodium and lamellum they usually disappeared,  F-actin underwent slower retrograde flow in the lamellum, and F-actin generally moved forward in the cell body. Time-lapse FSM was performed on a spinning disk confocal microscope system. Light from a 50 mW Krypton-Argon ion laser (Melles Griot, OmniChrome) was delivered by a single-mode fiber optic (Point Source) to a Yokogawa spinning disk confocal scan-head (Ultra-View; PerkinElmer) on an inverted microscope (TE300 Quantum; Nikon).  Images were collected by a 100x 1.4NA Plan-Apo DIC objective lens (Nikon) and captured with an Orca 2 camera (Hamamatsu). Images were collected in the ventral focal plane at 10 s intervals.  Images were processed as follows: (1) background subtraction; (2) 3 x3 low pass filter; (3) unsharp mask filter. Corresponds to Fig 2E and video 2 in JCB , Volume 158, Number 1, July 8, 2002 31-37"},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"actin cytoskeleton","onto_id":"GO:0015629"},"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"3420","DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},{"free_text":"fluorescent speckle microscopy"}],"DIMENSION":[{"Space":{"Image_size":371,"Pixel_size":{"unit":"microns","value":0.119},"axis":"X"}},{"Space":{"Image_size":337,"Pixel_size":{"unit":"microns","value":0.119},"axis":"Y"}},{"Time":{"unit":"seconds","value":10,"frame":"25min 34 s"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Taricha granulosa","onto_id":"NCBITaxon:8321"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"X-Rhodamine","onto_id":"FBbi:00000584"},"CELLTYPE":{"onto_name":"epithelial cell of lung","onto_id":"CL:0000082"}}},"Citation":{"Title":"Wendy C. Salmon, Michael C. Adams, Clare M. Waterman-Storer (2011) CIL:11932, Taricha granulosa, epithelial cell of lung. CIL. Dataset","ARK":"ark:\/b7295\/w9cil11932","DOI":"doi:10.7295\/W9CIL11932"}}}