CIL:7917, Mus musculus
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- Collection
- Cite This Work
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Ghosh, Rajarshi; Woodcock, Christopher (2021). CIL:7917, Mus musculus. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0BG2N00
- Description
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Fluorescence recovery after photobleaching (FRAP)kinetics of histone H1-zero were recorded using mouse fibroblast cell line stably expressing GFP-H1-zero. Live cells were imaged using a Zeiss LSM 510 meta confocal microscope with fluorescence excitation at 488 nm and a 63x plan-apo oil lens. The image series has 2 pre-bleach shots, one immediately after bleaching a 1 micrometer diameter circle of pericentromeric heterochromatin, and 47 images at 5 sec intervals.
- Date Issued
- 2021
- Researchers
- Technical Details
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Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: quantity of GFP-protein
Visualization methods: Green fluorescent proteins from Anthozoa
Processing history: unprocessed raw data - Series
- Scientific Name
- Anatomy
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- No linguistic content; Not applicable
- Identifier
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Samplenumber: 7917
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7917
- Ghosh RP, Horowitz-Scherer RA, Nikitina T, Shlyakhtenko LS, Woodcock CL. MeCP2 binds cooperatively to its substrate and competes with histone H1 for chromatin binding sites. Mol Cell Biol. 2010 Oct;30(19):4656-70. https://doi.org/10.1128/MCB.00379-10
- PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=11130729
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12