{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1285646400},"Data_type":{"Still_image":false,"Z_stack":false,"Video":false,"Time_series":true},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"7917.zip","Size":15626740,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"7917.jpg","Size":48567,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"7917.tif","Size":13200000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"Contributors":["Ghosh, Rajarshi","Woodcock, Chris"],"PUBMED":["20679481","11130729"]},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Fluorescence recovery after photobleaching (FRAP)kinetics of histone H1-zero were recorded using mouse fibroblast cell line stably expressing GFP-H1-zero. Live cells were imaged using a Zeiss LSM 510 meta confocal microscope with fluorescence excitation at 488 nm and a 63x plan-apo oil lens. The image series has 2 pre-bleach shots, one immediately after bleaching a 1 micrometer diameter circle of pericentromeric heterochromatin, and 47 images at 5 sec intervals."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"nuclear chromatin","onto_id":"GO:0000790"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"free_text":"quantity of GFP-protein"},"GROUP_ID":"1513","MOLECULARFUNCTION":{"free_text":"mobility of nuclear protein"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"DIMENSION":[{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.05},"axis":"X"}},{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.05},"axis":"Y"}},{"Time":{"unit":"seconds","value":5,"frame":"50"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"Green fluorescent proteins from Anthozoa","onto_id":"FBbi:00000481"},"CELLTYPE":{"onto_id":"MC:BALB\/c 3T3 fibroblast"}}},"Citation":{"Title":"Ghosh, Rajarshi, Woodcock, Chris (2010) CIL:7917, Mus musculus. CIL. Dataset","ARK":"ark:\/b7295\/w9cil7917","DOI":"doi:10.7295\/W9CIL7917"}}}