Perlstein, Ethan; Korostyshevsky, Daniel (2021). CIL:40436, Saccharomyces cerevisiae. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0PZ57P2

Transmission electron micrograph of a representative untreated swa2 mutant cell. SWA2 encodes the yeast homolog of auxilin, which is a co-chaperone that regulates clathrin assembly and disassembly. swa2 mutant cells exhibit a vacuolar fragmentation phenotype. (6,300X). An image of the same mutant following sertraline treatment is available as CIL 40435.

• 2021

• Korostyshevsky, Daniel
• Perlstein, Ethan

This group of images contains representative whole-cell images and high-magnification zooms of organelle structures of interest. All cells were grown in liquid rich media (YPD pH6.5) at 30C with aerated shaking, and cultures were sampled in mid-logarithmic phase. Treated cells were exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes.

Preparation: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data

• Cell Image Library Group ID: 11320

• Saccharomyces cerevisiae

• Nucleus
• Vacuole

• Organelle organization
• Vacuole fragmentation

• data
• image

• No linguistic content; Not applicable

Samplenumber: 40436

Doi: https://doi.org/10.6075/J0PZ57P2

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40436

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12