{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1333080000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"40436.tif","Size":6100000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"40436.zip","Size":4373578,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"40436.jpg","Size":1296742,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"OTHER":["Princeton EM Core Facility"],"Contributors":["Ethan Perlstein","Daniel Korostyshevsky"]},"BIOLOGICALPROCESS":[{"onto_name":"organelle organization","onto_id":"GO:0006996"},{"free_text":"vacuole fragmentation"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Transmission electron micrograph of a representative untreated swa2 mutant cell. SWA2 encodes the yeast homolog of auxilin, which is a co-chaperone that regulates clathrin assembly and disassembly. swa2 mutant cells exhibit a vacuolar fragmentation phenotype. (6,300X).  An image of the same mutant following sertraline treatment is available as CIL 40435."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"vacuole","onto_id":"GO:0005773"},{"onto_name":"nucleus","onto_id":"GO:0005634"}],"RELATIONTOINTACTCELL":{"onto_name":"sectioned tissue","onto_id":"FBbi:00000026"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"11320","TECHNICALDETAILS":{"free_text":"This group of images contains representative whole-cell images and high-magnification zooms of organelle structures of interest. All cells were grown in liquid rich media (YPD pH6.5) at 30C with aerated shaking, and cultures were sampled in mid-logarithmic phase. Treated cells were exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes."},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":2576,"axis":"X"}},{"Space":{"Image_size":2328,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Saccharomyces cerevisiae","onto_id":"NCBITaxon:4932"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"tissue in plastic embedment","onto_id":"FBbi:00000016"}],"VISUALIZATIONMETHODS":{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"}}},"Citation":{"Title":"Ethan Perlstein, Daniel Korostyshevsky (2012) CIL:40436, Saccharomyces cerevisiae. CIL. Dataset","ARK":"ark:\/b7295\/w9cil40436","DOI":"doi:10.7295\/W9CIL40436"}}}