Antipa, Gregory (2021). CIL:25691, Trichodina, Chlorella, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0C53JSN

A view of the cytoplasm of a green Trichodina that lives on the surface of a freshwater snail collected in central Illinois. In this view the 10 endosymbiotic bacteria are evident as well as an endosymbiotic zoochlorella. The endosymbiotic bacteria are full of viral particles and a few can be seen in the cytoplasm. A large food vacuole is present and oral membranelles are evident. Other features of the oral apparatus such as microtubules aligning the walls and a noded system at one side are exhibited. Additionaly, the macronuclear organization is observed. TEM taken by G. Antipa in 1968 on a Hitachi HU11A operating at 75kV. The negative magnification is 5,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 65nm thickness.

• 2021

• Antipa, Gregory

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw

• Chlorella
• Trichodina

• Bacterial endosymbiont
• Cell by organism
• Ciliated Protist
• Cytoplasm
• Eukaryotic cell
• Eukaryotic Protist
• Food vacuole
• Macronucleus
• Microtubule associated complex
• Noded fiber
• Oral apparatus
• Symbiont-containing vacuole
• Zoochlorellae

• Cytoplasm organization
• Eukaryotic photosynthetic symbiont
• Macronucleus organization
• Oral apparatus organization
• Prokaryotic symbiont
• Response to symbiont
• Virion transport

• data
• image

• No linguistic content; Not applicable

Samplenumber: 25691

Doi: https://doi.org/10.6075/J0C53JSN

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL25691

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12