Cammer, Michael; Cox, Dianne (2021). CIL:35142, Mus musculus, macrophage. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J01R6PG5

Phase contrast microscopy of living bone marrow derived macrophages. These cells are highly motile and the snapshot catches lamella and ruffles where the cells are protruding and phase dense organelles inside the volumes of the cells.

• 2021

• Cammer, Michael
• Cox, Dianne

Cells were extracted from a mouse femur and plated in a coverslip bottom well (MatTek) in standard culture media. The Olympus IX 71 microscope had a simple heated stage. Cells were imaged with a 60X N.A. 1.4 phase 3 planapo objective with a Cooke Sensicam QE operated by IP Lab software running on a Dell Windows XP computer.

This picture is one of a series that may be composed into a montage to see a large field of cells interacting while preserving the high magnification. Some of the pixels in this and the neighboring images are saturated.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 8397

• Mus musculus

• Macrophage
• Ruffle membrane
• TS24,femur

• Cell motility
• Macrophage

• data
• image

• No linguistic content; Not applicable

Samplenumber: 35142

Doi: https://doi.org/10.6075/J01R6PG5

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35142

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2022-08-12