{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1306987200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"35142.zip","Size":2870736,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"35142.jpg","Size":140532,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"35142.tif","Size":2900000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"Contributors":["Michael Cammer","Dianne Cox"]},"BIOLOGICALPROCESS":{"onto_name":"cell motility","onto_id":"GO:0048870"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"Macrophage","onto_id":"FMA:63261"},"PARAMETERIMAGED":{"onto_name":"elastic scattering of photons","onto_id":"FBbi:00000587"},"IMAGEDESCRIPTION":{"free_text":"Phase contrast microscopy of living bone marrow derived macrophages.  These cells are highly motile and the snapshot catches lamella and ruffles where the cells are protruding and phase dense organelles inside the volumes of the cells."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"ruffle membrane","onto_id":"GO:0032587"},"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"differences in amount of elastic light scattering","onto_id":"FBbi:00000600"},"GROUP_ID":"8397","TECHNICALDETAILS":{"free_text":"Cells were extracted from a mouse femur and plated in a coverslip bottom well (MatTek) in standard culture media.  The Olympus IX 71 microscope had a simple heated stage.  Cells were imaged with a 60X N.A. 1.4 phase 3 planapo objective with a Cooke Sensicam QE operated by IP Lab software running on a Dell Windows XP computer.\n\nThis picture is one of a series that may be composed into a montage to see a large field of cells interacting while preserving the high magnification.  Some of the pixels in this and the neighboring images are saturated."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"phase contrast microscopy","onto_id":"FBbi:00000247"},"DIMENSION":[{"Space":{"Image_size":1376,"Pixel_size":{"unit":"microns","value":0.11},"axis":"X"}},{"Space":{"Image_size":1040,"Pixel_size":{"unit":"microns","value":0.11},"axis":"Y"}}],"MOUSE_GROSS_ANATOMY":[{"onto_name":"TS24,femur","onto_id":"EMAP:12912"}],"SPECIESTAXASPECIFIC":{"onto_id":"EMAP:EMAP:12912"},"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"},"CELLTYPE":{"onto_name":"macrophage","onto_id":"CL:0000235"},"PATHOLOGICALPROCESS":{"onto_id":"IDO:IDO:0000012"}}},"Citation":{"Title":"Michael Cammer, Dianne Cox (2011) CIL:35142, Mus musculus, macrophage. CIL. Dataset","ARK":"ark:\/b7295\/w9cil35142","DOI":"doi:10.7295\/W9CIL35142"}}}