Allen, Richard (2021). CIL:9685, Coleps hirtus, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0PZ581Z

Cross section of a Coleps cilium showing the axoneme consisting of 9 doublet and 2 central microtubules. Doublets have a complete A subfiber and an attached B subfiber. Two dynein arms, outer and inner, extend from the A subfiber. The ciliary membrane with a dense external coat surrounds the ciliary axoneme. The plasma membrane has a glycocalyx composed of the dense coat plus longer filaments. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 5/28/69 by R. Allen with Philips 300 operating at 60kV. Neg. 92,500X. The negative was printed to paper and the image was scanned with a flatbed scanner and 100nm bar added in Photoshop. This digitized image is available for qualitative analysis. A raw, unprocessed, high resolution version of this image (CIL:2871) is in the library and available for quantitative analysis.

Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: stain with broad specificity; recorded image; film; Print from negative scanned to Photoshop.
Data qualification: Processed;spatialmeasurements

• Cell Image Library Group ID: 3267

• Coleps hirtus

• Axonemal dynein complex
• Axonemal microtubule
• Ciliated Protist
• Cilium axoneme
• Eukaryotic cell
• Eukaryotic Protist
• Glycocalyx

• Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
• Ciliary or flagellar motility
• Protein galactosylation at cell surface
• Structural molecule activity

• data
• image

• No linguistic content; Not applicable

Samplenumber: 9685

Doi: https://doi.org/10.6075/J0PZ581Z

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL9685

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12