{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1289797200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"9685.zip","Size":14204698,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"9685.tif","Size":14300000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"9685.jpg","Size":1798457,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Allen"]},"BIOLOGICALPROCESS":[{"onto_name":"ciliary or flagellar motility","onto_id":"GO:0001539"},{"onto_name":"protein galactosylation at cell surface","onto_id":"GO:0033580"}],"PROCESSINGHISTORY":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"free_text":"Print from negative scanned to Photoshop."}],"CELLLINE":{"free_text":"Cambridge Culture Collection, Cambridge, England, catalog # LB1656\/1"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Cross section of a Coleps cilium showing the axoneme consisting of 9 doublet and 2 central microtubules. Doublets have a complete A subfiber and an attached B subfiber. Two dynein arms, outer and inner, extend from the A subfiber. The ciliary membrane with a dense external coat surrounds the ciliary axoneme. The plasma membrane has a glycocalyx composed of the dense coat plus longer filaments. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 5\/28\/69 by R. Allen with Philips 300 operating at 60kV. Neg. 92,500X. The negative was printed to paper and the image was scanned with a flatbed scanner and 100nm bar added in Photoshop. This digitized image is available for qualitative analysis. A raw, unprocessed, high resolution version of this image (CIL:2871) is in the library and available for quantitative analysis.\n\nAdditional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"cilium axoneme","onto_id":"GO:0035085"},{"onto_name":"axonemal microtubule","onto_id":"GO:0005879"},{"onto_name":"axonemal dynein complex","onto_id":"GO:0005858"},{"onto_name":"glycocalyx","onto_id":"GO:0030112"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"3267","MOLECULARFUNCTION":{"onto_name":"structural molecule activity","onto_id":"GO:0005198"},"DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":3000,"axis":"X"}},{"Space":{"Image_size":2364,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Coleps hirtus","onto_id":"NCBITaxon:60000"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (2010) CIL:9685, Coleps hirtus, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil9685","DOI":"doi:10.7295\/W9CIL9685"}}}