Nishimura, Yukako; Waterman, Clare M. (2021). CIL:7438, Homo sapiens, fibroblast, primary cell line cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0P55MG0

Human foreskin fibroblasts (HFF1) cells transfected with mKusabira-Orange EB3, a marker for growing microtubule ends. Note the dramatic movement of the bright fluorescent markers toward the cell periphery.

Cells were imaged every 3 s by a spinning disk confocal microscoe on a Nikon Ti2000 using a 1.4NA Plan Apo
Ex objective and a solid state 568 laser with a 605x52nm EM filter (Semrock). Images were recorded with a Coolsnap HQ2 (Photometrics) using 500 ms exposures.

• 2021

• Nishimura, Yukako
• Waterman, Clare M.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Kusabira OrangeFP
Processing history: unprocessed raw data

• Homo sapiens

• Fibroblast
• Microtubule plus end
• Primary cell line cell

• Human foreskin fibroblast (HFF)
• Microtubule plus-end binding
• Microtubule polymerization
• Prepuce of penis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7438

Doi: https://doi.org/10.6075/J0P55MG0

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7438

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12