{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1288929600},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"7438.zip","Size":234649661,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"7438.tif","Size":234900000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"7438.jpg","Size":151857,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"OTHER":["NHLBI\/NIH"],"Contributors":["Yukako Nishimura","Clare M. Waterman"]},"BIOLOGICALPROCESS":{"onto_name":"microtubule polymerization","onto_id":"GO:0046785"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":[{"onto_name":"Prepuce of penis","onto_id":"FMA:19639"},{"free_text":"human foreskin fibroblast (HFF)"}],"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Human foreskin fibroblasts (HFF1) cells transfected with mKusabira-Orange EB3, a marker for growing microtubule ends.  Note the dramatic movement of the bright fluorescent markers toward the cell periphery.\n\nCells were imaged every 3 s by a spinning disk confocal microscoe on a Nikon Ti2000 using a 1.4NA Plan Apo\nEx objective and a solid state 568 laser with a 605x52nm EM filter (Semrock).  Images were recorded with a Coolsnap HQ2 (Photometrics) using 500 ms exposures."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"microtubule plus end","onto_id":"GO:0035371"},"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"MOLECULARFUNCTION":{"onto_name":"microtubule plus-end binding","onto_id":"GO:0051010"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},"DIMENSION":[{"Space":{"Image_size":1392,"Pixel_size":{"unit":"microns","value":0.105},"axis":"X"}},{"Space":{"Image_size":1040,"Pixel_size":{"unit":"microns","value":0.105},"axis":"Y"}},{"Time":{"unit":"seconds","value":3}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"Kusabira OrangeFP","onto_id":"FBbi:00000512"},"CELLTYPE":[{"onto_name":"fibroblast","onto_id":"CL:0000057"},{"onto_name":"primary cell line cell","onto_id":"CL:0000001"}]}},"Citation":{"Title":"Yukako Nishimura, Clare M. Waterman (2010) CIL:7438, Homo sapiens, fibroblast, primary cell line cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil7438","DOI":"doi:10.7295\/W9CIL7438"}}}