von Dassow, George (2021). CIL:15810, Strongylocentrotus purpuratus. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09022J0

Purple sea urchin embryos undergoing mitosis. Embryos are expressing GFP-rhotekin GTPase binding domain, which is a probe for active Rho, with 3xmCherry-Ensconsin (a microtubule asociated protein shown in blue). Embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14–17°C

• 2021

• Von Dassow, George

Preparation: unfixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP; mCherryFP
Processing history: unprocessed raw data

• Strongylocentrotus purpuratus

• Astral microtubule
• Ensconsin
• Microtubule
• Spindle

• Active Rho
• Embryo
• Mitotic anaphase
• Mitotic metaphase
• Mitotic telophase

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 15810

Doi: https://doi.org/10.6075/J09022J0

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL15810

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2022-08-12