{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1301716800},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"15810.zip","Size":3166336,"Mime_type":"application\/zip"},{"File_type":"Mp4","File_path":"15810_web.mp4","Size":558463,"Mime_type":"video\/mp4"},{"File_type":"Jpeg","File_path":"15810.jpg","Size":58055,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["George Von  Dassow"]},"BIOLOGICALPROCESS":[{"onto_name":"mitotic metaphase","onto_id":"GO:0000089"},{"onto_name":"mitotic anaphase","onto_id":"GO:0000090"},{"onto_name":"mitotic telophase","onto_id":"GO:0000093"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"Embryo","onto_id":"FMA:69068"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Purple sea urchin embryos undergoing mitosis.  Embryos are expressing GFP-rhotekin GTPase binding domain, which is a probe for active Rho, with 3xmCherry-Ensconsin (a microtubule asociated protein shown in blue). Embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14\u201317°C"},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"astral microtubule","onto_id":"GO:0000235"},{"onto_name":"spindle","onto_id":"GO:0005819"},{"onto_name":"microtubule","onto_id":"GO:0005874"},{"free_text":"ensconsin"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"MOLECULARFUNCTION":{"free_text":"active Rho"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":480,"Pixel_size":{"unit":"microns","value":0.21},"axis":"X"}},{"Space":{"Image_size":480,"Pixel_size":{"unit":"microns","value":0.21},"axis":"Y"}},{"Time":{"unit":"seconds","value":12,"frame":"92"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Strongylocentrotus purpuratus","onto_id":"NCBITaxon:7668"},"PREPARATION":{"onto_name":"unfixed tissue","onto_id":"FBbi:00000014"},"VISUALIZATIONMETHODS":[{"onto_name":"EGFP","onto_id":"FBbi:00000082"},{"onto_name":"mCherryFP","onto_id":"FBbi:00000525"}]}},"Citation":{"Title":"George Von  Dassow (2011) CIL:15810, Strongylocentrotus purpuratus. CIL. Dataset","ARK":"ark:\/b7295\/w9cil15810","DOI":"doi:10.7295\/W9CIL15810"}}}