Leomhardt, Heinrich; Rahn, Hans-Peter; Weinziert, Peter; Sporbert, Anje; Cremer, Thomas; Zink, Daniele; Cordoso, M. Cristina (2021). CIL:39253, Mus musculus, myoblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J04M9367

Mouse myoblast cells were engineered to express GFP-PCNA (proliferating cell nuclear antigen) as a marker for replication factories, and also immunostained for DNA ligase. DNA was localized using Hoechst staining. The image shows two different typical S-phase patterns displaying GFP-PCNA (left), DNA ligase (center) and DNA (right). DNA ligase co-localizes with PCNA at replication foci.

• 2021

• Cordoso, M. Cristina
• Cremer, Thomas
• Leomhardt, Heinrich
• Rahn, Hans-Peter
• Sporbert, Anje
• Weinziert, Peter
• Zink, Daniele

C2C12 cells stably expressing GFP-PCNA were fixed,stained for Dnmt1 and DNA, and optical sections acquired with a Leica TCS confocal microscope.

See: Fig 2A in H Leonhardt et al. 2000 Dynamics of DNA replication factories in living cells. J Cell Biol 149:271-279

Preparation: formaldehyde fixed tissue; living tissue
Relation to intact cell: whole mounted tissue
Item type: photomultiplier tube (PMT); charge coupled device (CCD)
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: Fluorescein (FITC); EGFP
Processing history: montage
Data qualification: Processed

• Cell Image Library Group ID: 10204

• Mus musculus

• Myoblast
• Nuclear chromatin
• Nuclear DNA

• C2C12
• DNA metabolic process
• DNA replication

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39253

Doi: https://doi.org/10.6075/J04M9367

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39253

Reference

• H Leonhardt et al. 2000 Dynamics of DNA replication factories in living cells. J Cell Biol 149:271-279 https://www.ncbi.nlm.nih.gov/pubmed/?term=10769021

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2022-08-12