{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1325048400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"39253.jpg","Size":15767,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"39253.zip","Size":312746,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"39253.tif","Size":400000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["H Leonhardt et al. 2000 Dynamics of DNA replication factories in living cells. J Cell Biol 149:271-279"],"Contributors":["Heinrich Leomhardt","Hans-Peter Rahn","Peter Weinziert","Anje Sporbert","Thomas Cremer","Daniele Zink","M Cristina Cordoso"],"PUBMED":["10769021"]},"BIOLOGICALPROCESS":[{"onto_name":"DNA replication","onto_id":"GO:0006260"},{"onto_name":"DNA metabolic process","onto_id":"GO:0006259"}],"PROCESSINGHISTORY":{"onto_name":"montage","onto_id":"FBbi:00000261"},"CELLLINE":{"onto_name":"C2C12","onto_id":"MCC:0000079"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Mouse myoblast cells were engineered to express GFP-PCNA (proliferating cell nuclear antigen) as a marker for replication factories, and also immunostained for DNA ligase. DNA was localized using Hoechst staining.  The image shows two different typical S-phase patterns displaying GFP-PCNA (left), DNA ligase (center) and DNA (right). DNA ligase co-localizes with PCNA at replication foci."},"ITEMTYPE":[{"onto_name":"photomultiplier tube (PMT)","onto_id":"FBbi:00000295"},{"onto_name":"charge coupled device (CCD)","onto_id":"FBbi:00000294"}],"CELLULARCOMPONENT":[{"onto_name":"nuclear chromatin","onto_id":"GO:0000790"},{"free_text":"nuclear DNA"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"},"GROUP_ID":"10204","TECHNICALDETAILS":{"free_text":"C2C12 cells stably expressing GFP-PCNA were fixed,stained for Dnmt1 and DNA, and optical sections acquired with a Leica TCS confocal microscope.  \n\nSee: Fig 2A in H Leonhardt et al. 2000 Dynamics of DNA replication factories in living cells. J Cell Biol 149:271-279"},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":390,"axis":"X"}},{"Space":{"Image_size":267,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"living tissue","onto_id":"FBbi:00000025"}],"VISUALIZATIONMETHODS":[{"onto_name":"Fluorescein (FITC)","onto_id":"FBbi:00000451"},{"onto_name":"EGFP","onto_id":"FBbi:00000082"}],"CELLTYPE":{"onto_name":"myoblast","onto_id":"CL:0000056"}}},"Citation":{"Title":"Heinrich Leomhardt, Hans-Peter Rahn, Peter Weinziert, Anje Sporbert, Thomas Cremer, Daniele Zink, M Cristina Cordoso (2011) CIL:39253, Mus musculus, myoblast. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39253","DOI":"doi:10.7295\/W9CIL39253"}}}