Biais, Nicolas; Ladoux, Benoît; Higashi, Dustin; So, Magdalene; Sheetz, Michael (2021). CIL:34523, Neisseria gonorrhoeae, motile cell, bacteria. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0QF8RJR

The video is a representative movie of baceria incubated in DMEM and polylysine for 3 h on top of an array of pilars of stiffness 500 pN/micron. The video is real time and shows the behavior of one or two bacteria for 23 s after 3 h of incubation. The video relates to CIL: 34521 and 34522, which show bacteria incubated in different media. The pilars are 3 micron apart center to center. The video is DIC microscopy with the focus on the top of the pilars. See the absence of motions in this media. This video corresponds to Video 4 from PLoS Biol. 2008 Apr 15;6(4):e87.

• 2021

• Biais, Nicolas
• Higashi, Dustin
• Ladoux, Benoît
• Sheetz, Michael
• So, Magdalene

A pulling force exerted on a pillar, such as that imposed by an attached pilus fiber, causes a displacement of the pillar tip. The displacement can be correlated to the force applied on the pillar after proper calibrations. All optical microscope images were obtained on conventional inverted microscopes (either Olympus IX 71 or Zeiss Axiovert 100).

Preparation: whole mounted tissue
Relation to intact cell: living tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy; force microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data

• Cell Image Library Group ID: 8217

• Neisseria gonorrhoeae

• Bacteria
• Motile cell
• Type IV pilus

• Retraction force

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 34523

Doi: https://doi.org/10.6075/J0QF8RJR

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL34523

Reference

• PLoS Biol. 2008 Apr 15;6(4):e87. https://www.ncbi.nlm.nih.gov/pubmed/?term=18416602

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12