{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1306036800},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"34523.zip","Size":6271104,"Mime_type":"application\/zip"},{"File_type":"Mp4","File_path":"34523_web.mp4","Size":1847557,"Mime_type":"video\/mp4"},{"File_type":"Jpeg","File_path":"34523.jpg","Size":10546,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["PLoS Biol. 2008 Apr 15;6(4):e87."],"Contributors":["Nicolas Biais","Benoît Ladoux","Dustin Higashi","Magdalene So","Michael Sheetz"],"PUBMED":["18416602"]},"BIOLOGICALPROCESS":{"free_text":"retraction force"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"optical path length gradient","onto_id":"FBbi:00000312"},"IMAGEDESCRIPTION":{"free_text":"The video is a representative movie of baceria incubated in DMEM and polylysine for 3 h on top of an array of pilars of stiffness 500 pN\/micron. The video is real time and shows the behavior of one or two bacteria for 23 s after 3 h of incubation. The video relates to CIL: 34521 and 34522, which show bacteria incubated in different media.  The pilars are 3 micron apart center to center. The video is DIC microscopy with the focus on the top of the pilars. See the absence of motions in this media.  This video corresponds to Video 4 from PLoS Biol. 2008 Apr 15;6(4):e87."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"type IV pilus","onto_id":"GO:0044096"},"RELATIONTOINTACTCELL":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"SOURCEOFCONTRAST":{"onto_name":"boundaries between regions with different refractive index","onto_id":"FBbi:00000599"},"GROUP_ID":"8217","TECHNICALDETAILS":{"free_text":"A pulling force exerted on a pillar, such as that imposed by an attached pilus fiber, causes a displacement of the pillar tip.  The displacement can be correlated to the force applied on the pillar after proper calibrations.  All optical microscope images were obtained on conventional inverted microscopes (either Olympus IX 71 or Zeiss Axiovert 100)."},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"differential interference contrast microscopy","onto_id":"FBbi:00000245"},{"free_text":"force microscopy"}],"DIMENSION":[{"Space":{"Image_size":200,"axis":"X"}},{"Space":{"Image_size":200,"axis":"Y"}},{"Time":{"unit":"seconds","value":0.033,"frame":"700"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Neisseria gonorrhoeae","onto_id":"NCBITaxon:485"},"PREPARATION":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"VISUALIZATIONMETHODS":{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"},"CELLTYPE":[{"onto_name":"motile cell","onto_id":"CL:0000219"},{"free_text":"bacteria"}]}},"Citation":{"Title":"Nicolas Biais, Benoît Ladoux, Dustin Higashi, Magdalene So, Michael Sheetz (2011) CIL:34523, Neisseria gonorrhoeae, motile cell, bacteria. CIL. Dataset","ARK":"ark:\/b7295\/w9cil34523","DOI":"doi:10.7295\/W9CIL34523"}}}