Crammer, Michael (2021). CIL:34524, Homo sapiens, sperm. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0WS8S74

Human sperm diluted in buffer imaged at room temperature within two hours of ejaculation. Cell at center swims at constant speed.

• 2004-07-01

• 2021

• Crammer, Michael

Sperm were diluted with PBS and plated in a coverslip bottom chamber on an Olympus IX70 with 60X N.A. 1.4 phase 3 optics. Images were collected with an air cooled CCD camera (either Roper Coolsnap or Cooke Sensicam) at 12 bits and converted to 8 bits. The time interval was less than 1 second per image.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Processing history: living tissue
Data qualification: Processed;spatialmeasurements

• Homo sapiens

• Flagellum
• Gonadal component
• Sperm

• Ciliary or flagellar motility
• Mature sperm cell
• Sperm motility

• data
• image

• No linguistic content; Not applicable

Samplenumber: 34524

Doi: https://doi.org/10.6075/J0WS8S74

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL34524

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2023-08-17